The ACAS database organizes experiment data (experiment parameters, raw image input, processed images, analysis data, final reports) so that it is easy to understand results and correlate them to your experiment setup.

Projects

A project is a collection of experiments.  ACAS does not impose any rules or requirements that connect the experiments in the project. It is up to the user how to organize and define projects. However, ACAS does require that all replicates in an experiment have the same parameters.

For multi-user subscriptions to ACAS, projects have the benefit of restricting access to specific users. The project creator can grant access to team members.  This can be very useful in large organizations or university settings to protect data from accidental deletion or editing.

Experiments

Experiments are collections of replicates where each replicate has the same parameters (same assay type, plate type, etc). Conceptually, the set contains repeated trials of the same experiment such that the results can be statistically averaged.

Replicate

A replicate is a collection of wells (or a single well) from a multi-well plate.

Wells

A well contains a user-supplied image or image set (in the case of time-lapse). The analysis will start automatically soon after the raw image is uploaded to the well. After analysis, the well will also contain the tracked images as well as the track data file associated with the input images.

In this video, you can see how to download the analysis data from a well and also view the final results of each well in the report.

Wells can also be deleted and created one-at-a-time. Note, if not using the experiment wizard to bulk upload, folders cannot be drag-and-dropped into the well. A new report will be auto-generated once the new well has been processed and filled with analyzer output files. This video will show you how to create a new well and browse to your images to upload them: