Sample Track Movie

Assay Description

This assay is optimized for bright field images of moving cells such as T-Cells. Choose this version of chemotaxis assay if your image and cells have similar characteristics as the image below.

Microscopy Requirements

Microscopy Imaging Mode
Microscopy Resolution
Optimal: 2.0 pixels/µm or higher; Minimum: 0.5 pix/µm
Field of View
Approximately 1.5 mm x 2.0 mm
Time Between Frames
XX seconds to XX seconds
Image Size
Typically between 1024 and 2048 pixels wide
Image Aspect Ratio
Acceptable range is 4:3 to 16:9
Compatible Image Formats
jpg, png, bmp, tif
Compatible Video Formats
mp4, avi, mov, multi-frame-tif
Cell Density
60 / 1.0 mm2
Typical Cell Size
8.6 µm with a max extension of 20 µm

Example Image

Typical Cell Size 19 x 19 pixels (8.6 µm)
Image Size 1392 x 1040 pixels (636 µm x 475 µm)
Image Resolution 2.187 pixels/µm

(tech note on calculating microscopy resolution)

Chemotaxis BF10 Assay Guide Small Sample Image
Chemotaxis BF10 Assay Guide Sample Image

Try It Yourself

Upload the sample movies into your free ACAS account and you will get the same results as in this sample report.

Sample Time-lapse Movies for Upload
Click here to download sample movies. Be sure to extract the zip file before uploading to your ACAS account.