In this movie, the cell density is too high and extensive cell-to-cell contacts break the track lengths into small segments. As the cell density drops, the duration of the tracks increases as well as track accuracy.
Click the image below to see it full size. The image size is 1500 x 800 pixels. The slide was an ibidi µ-Slide chemotaxis chamber. The chamber walls are 1 mm apart and can be used as a reference to validate the microscopy resolution.
The microscopy resolution can be found by noting the distance between the chamber walls is 1000 µm and the number of pixels over this same distance is 620. So the resolution is 620 pix/1000 µm = 0.620 pix / µm (tech note on calculating microscopy resolution).
The cell sizes range from 20 to 50 µm, considering only the main body of the cells (excluding the extended pseudopodia).
Try It Yourself
Upload the sample movies into your free ACAS account and you will get the same results as in this sample report.
Click here to download sample movies. Be sure to extract the zip file before uploading to your ACAS account.