In this movie, the cell density is too high and extensive cell-to-cell contacts break the track lengths into small segments. As the cell density drops, the duration of the tracks increases as well as track accuracy.

Assay Description

This assay is optimized for phase contrast images of moving cells such as fibroblasts. Choose this version of chemotaxis assay if your image and cells have similar characteristics as the image below.

Microscopy Requirements

Microscopy Imaging Mode
Microscopy Resolution
Optimal: 2.0 pixels/µm; Minimum: 0.5 pix/µm
Field of View
Approximately 1.2 mm x 2 mm
Time Between Frames
10 seconds to 120 seconds
Image Size
Typically between 1024 and 2048 pixels wide
Image Aspect Ratio
Acceptable range is 4:3 to 16:9
Compatible Image Formats
jpg, png, bmp, tif
Compatible Video Formats
mp4, avi, mov, multi-frame-tif
Cell Density
60 to 100 per 1.0 mm2
Typical Cell Size
30 µm with a max extension of 50 µm

Example Image

Click the image below to see it full size. The image size is 1500 x 800 pixels. The slide was an ibidi µ-Slide chemotaxis chamber. The chamber walls are 1 mm apart and can be used as a reference to validate the microscopy resolution.

The microscopy resolution can be found by noting the distance between the chamber walls is 1000 µm and the number of pixels over this same distance is 620. So the resolution is 620 pix/1000 µm = 0.620 pix / µm (tech note on calculating microscopy resolution).

The cell sizes range from 20 to 50 µm, considering only the main body of the cells (excluding the extended pseudopodia).

Chemotaxis PC3050 Assay Large Sample Image

Try It Yourself

Upload the sample movies into your free ACAS account and you will get the same results as in this sample report.

Sample Time-lapse Movies for Upload

Click here to download sample movies. Be sure to extract the zip file before uploading to your ACAS account.