Posted on Sep 3, 2018

A well may contain a single image, or it may contain a time-lapse sequence.

Supported file types for single images are .bmp, .jpg, .png, .tif, .tiff.

For many experiments, the well will contain a time sequence of images.

Supported file types for time-lapse sequences are:

a) a collection of single images with the file types .bmp, .jpg, .png, .tif, .tiff. If you supply a collection of single images, be sure that the order of the images corresponds with the time order when the files are sorted alpha-numerically. On a Microsoft operating system, after sorting files by 'name', the files should be in time sequence where time zero is the first file.

b) a movie or multi-frame container such as .mp4, .tif, .tiff, .avi, .mov.

How to Upload a Collection of Frames

Generally, microscope recording systems will output a series of bitmaps or tif files. Tif files can contain a single image or a sequence of images.

upload images or moview

Any of these formats can be uploaded to the well. First determine if your image set is already contained in a single file (for example, mp4, avi, mov, or multi-frame tif). If so you can just directly upload these movie files to the well. Note, only one movie file per well.

If your image sequence is not contained in a movie file, you can also easily upload this sequence of images using the 'multi-image selection' button on the file uploader.

example multi-frame select

Now click "Choose Files". Click on the first file, then hold the shift key and click on the last file in the list, then all files will be selected.

Note: be sure the image file names are alphabetical in time-sequence. The ACAS will create a time-sequence movie (mp4) from these images. It will first sort them alpha-numerically. For example, image_000.jpg, image_001.jpg, would be a good naming convention to use.

multiple file in a folder for upload

A well may contain a single image, or it may contain a time-lapse sequence.

Supported file types for single images are .bmp, .jpg, .png, .tif, .tiff.

For many experiments, the well will contain a time sequence of images.

Supported file types for time-lapse sequences are:

a) a collection of single images with the file types .bmp, .jpg, .png, .tif, .tiff. If you supply a collection of single images, be sure that the order of the images corresponds with the time order when the files are sorted alpha-numerically. On a Microsoft operating system, after sorting files by 'name', the files should be in time sequence where time zero is the first file.

b) a movie or multi-frame container such as .mp4, .tif, .tiff, .avi, .mov.

How to Upload a Collection of Frames

Generally, microscope recording systems will output a series of bitmaps or tif files. Tif files can contain a single image or a sequence of images.

upload images or moview

Any of these formats can be uploaded to the well. First determine if your image set is already contained in a single file (for example, mp4, avi, mov, or multi-frame tif). If so you can just directly upload these movie files to the well. Note, only one movie file per well.

If your image sequence is not contained in a movie file, you can also easily upload this sequence of images using the 'multi-image selection' button on the file uploader.

example multi-frame select

Now click "Choose Files". Click on the first file, then hold the shift key and click on the last file in the list, then all files will be selected.

Note: be sure the image file names are alphabetical in time-sequence. The ACAS will create a time-sequence movie (mp4) from these images. It will first sort them alpha-numerically. For example, image_000.jpg, image_001.jpg, would be a good naming convention to use.

multiple file in a folder for upload

Posted on Sep 3, 2018

Posted on Sep 3, 2018

My files are contained in a single folder.  Time-lapse.

In the following example, three time-lapse chemotaxis movies are in one single folder.  Each file has a unique name that will become the name of the well. The well name will be used in the reports to present your results. To upload your files, just select all files simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

folder organization time lapse single folder

My files are contained in a single folder.  Single-Frame (endpoint).

In the following example, three endpoint images (one for each well) are in one single folder.  Each file has a unique name that will become the name of the well. The well name will be used in the reports to present your results. To upload your files, just select all files simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

folder organization single frame all one folder

My files are organized by folders, one folder by well.  Time Lapse. 

In the following example, three wells have been record over time. The images for each well are in seperate folders, one folder for each well. The name of the folder will become the name of the well. In this example, the images are a sequence of png files. Rather than individual frames for each time point, these folders could also contain mp4, avi, multi-frame tif, or zip files.  To upload your files, just select all well folders simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

My files are organized by folders, one folder by well.  Single Frame.

In the following example, three wells have been imaged at a single point in time. The images for each well are each in their own folder, one folder per well. In this case, the name of the folder will become the name of the well.  To upload your files, just select all well folders simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

Complete Guide to Uploading Image Files:

My files are contained in a single folder.  Time-lapse.

In the following example, three time-lapse chemotaxis movies are in one single folder.  Each file has a unique name that will become the name of the well. The well name will be used in the reports to present your results. To upload your files, just select all files simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

folder organization time lapse single folder

My files are contained in a single folder.  Single-Frame (endpoint).

In the following example, three endpoint images (one for each well) are in one single folder.  Each file has a unique name that will become the name of the well. The well name will be used in the reports to present your results. To upload your files, just select all files simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

folder organization single frame all one folder

My files are organized by folders, one folder by well.  Time Lapse. 

In the following example, three wells have been record over time. The images for each well are in seperate folders, one folder for each well. The name of the folder will become the name of the well. In this example, the images are a sequence of png files. Rather than individual frames for each time point, these folders could also contain mp4, avi, multi-frame tif, or zip files.  To upload your files, just select all well folders simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

My files are organized by folders, one folder by well.  Single Frame.

In the following example, three wells have been imaged at a single point in time. The images for each well are each in their own folder, one folder per well. In this case, the name of the folder will become the name of the well.  To upload your files, just select all well folders simultaneously (holding the shift key while selecting the range with the mouse), or just select the parent folder. Selected files should be dragged into the drop box in file upload page.

Complete Guide to Uploading Image Files:

Posted on Sep 3, 2018

NOTE:  Internet Explorer 10 and 11 do not support multi-folder upload.  We recommend Chrome and Firefox for the best experience uploading many wells.

In this example, the parent folder is selected and dropped into the drop-box. But you can also drag individual files or folders. Its best to select and drag the first parent folder that contains all the files you want to upload.

NOTE:  Internet Explorer 10 and 11 do not support multi-folder upload.  We recommend Chrome and Firefox for the best experience uploading many wells.

In this example, the parent folder is selected and dropped into the drop-box. But you can also drag individual files or folders. Its best to select and drag the first parent folder that contains all the files you want to upload.