Posted on Sep 3, 2018

When making time-lapse movies in multiple fluorescent channels, how to format the data can be very confusing. Each microscope is different in what its software prefers to export.

The bottom line is we can work with pretty much anything except proprietary formats. But we have our preferences.

MetaVi Labs preference order is:

First Choice

Each channel is in a separate mp4 file (using H.264 compression internally). The images are gray-scale. Keeping the channels separate will provide more accuracy than pre-composited images. MetaVi Labs software will composite and colorize the images for display after the tracking.

Second Choice

Each channel is associated with one folder, and each folder has a collection of tiff files, one file per time point.

Third Choice

Each time point is contained in a multi-frame tiff file. Each file has one image from each of the channels. Each image is grey-scale.

When making time-lapse movies in multiple fluorescent channels, how to format the data can be very confusing. Each microscope is different in what its software prefers to export.

The bottom line is we can work with pretty much anything except proprietary formats. But we have our preferences.

MetaVi Labs preference order is:

First Choice

Each channel is in a separate mp4 file (using H.264 compression internally). The images are gray-scale. Keeping the channels separate will provide more accuracy than pre-composited images. MetaVi Labs software will composite and colorize the images for display after the tracking.

Second Choice

Each channel is associated with one folder, and each folder has a collection of tiff files, one file per time point.

Third Choice

Each time point is contained in a multi-frame tiff file. Each file has one image from each of the channels. Each image is grey-scale.

Posted on Sep 3, 2018

Creating a new experiment is a one step process, just drag and drop:

Creating a new experiment is a one step process, just drag and drop:

Posted on Sep 2, 2018

This page will help determine how to handle your single-image (end-point assay) files. Please read the two options and at the bottom of this page click on the link that best describes your situation.

Allowed single frame image formats are: png (preferred), jpg, bmp, tif.

Single Folder

All image files are contained in one folder. Each file is an image from a well. So if you have 96 wells, you have 96 image files in one folder. The image file name will become the well name in ACAS.

By Folder

Your files are organized with one folder for each well. Each folder contains one image file. The folder name will be the well name in ACAS.

So we can show you the correct demo video for your situation, please click on one of the blue arrows below:

single folder microscopy images
multiple folder microscopy images

This page will help determine how to handle your single-image (end-point assay) files. Please read the two options and at the bottom of this page click on the link that best describes your situation.

Allowed single frame image formats are: png (preferred), jpg, bmp, tif.

Single Folder

All image files are contained in one folder. Each file is an image from a well. So if you have 96 wells, you have 96 image files in one folder. The image file name will become the well name in ACAS.

By Folder

Your files are organized with one folder for each well. Each folder contains one image file. The folder name will be the well name in ACAS.

So we can show you the correct demo video for your situation, please click on one of the blue arrows below:

single folder microscopy images
multiple folder microscopy images

Posted on Sep 2, 2018

Sometimes something goes wrong with an experiment and MetaVi Labs' technical support specialists would like to figure out what is going wrong so we can get your experiment results back on track.

You can give permission to our tech support staff to access your account by clicking a check box in your personal account page. You can then disable access when the issue is resolved (or any time you choose).

So that Tech support can find your account, they will ask you for your customer account name, URL, and your login email address.  They will not ask for your password.

Step 1: Click the "Allow support access" check box

Step 2: click the "Save" button.

Step 3: email 'support@metavilabs.com" with  your customer account name, URL, and your login email address.

Here is a how-to-video which will demonstrate where to find the "Allow support access" checkbox:

Sometimes something goes wrong with an experiment and MetaVi Labs' technical support specialists would like to figure out what is going wrong so we can get your experiment results back on track.

You can give permission to our tech support staff to access your account by clicking a check box in your personal account page. You can then disable access when the issue is resolved (or any time you choose).

So that Tech support can find your account, they will ask you for your customer account name, URL, and your login email address.  They will not ask for your password.

Step 1: Click the "Allow support access" check box

Step 2: click the "Save" button.

Step 3: email 'support@metavilabs.com" with  your customer account name, URL, and your login email address.

Here is a how-to-video which will demonstrate where to find the "Allow support access" checkbox:

Posted on Sep 2, 2018

If you make a mistake entering a parameter, you may want to re-do the report on a specific experiment. You can do this at any time (at no cost) by clicking the re-do button. report icon

Note: reports will not be generated (and the re-do button will not appear) unless all wells have processed data from the analyzer. If you have an empty well and would like a report anyway, you can delete the empty well (you can always create a new well later).

The report re-do icon appears on two pages (at the experiment-set page and also the experiment page). Below you will see it on the experiment-set page:

If you make a mistake entering a parameter, you may want to re-do the report on a specific experiment. You can do this at any time (at no cost) by clicking the re-do button. report icon

Note: reports will not be generated (and the re-do button will not appear) unless all wells have processed data from the analyzer. If you have an empty well and would like a report anyway, you can delete the empty well (you can always create a new well later).

The report re-do icon appears on two pages (at the experiment-set page and also the experiment page). Below you will see it on the experiment-set page:

Posted on Sep 2, 2018

Note that items marked with a red * are required fields. All other fields are optional.

Once the first experiment is added to the set, the plate type and assay type will no longer be editable.

Note that items marked with a red * are required fields. All other fields are optional.

Once the first experiment is added to the set, the plate type and assay type will no longer be editable.

Posted on Sep 2, 2018

 

Advanced Guide to Working with Projects in ACAS

The ACAS database organizes experiment data (experiment parameters, raw image input, processed images, analysis data, final reports) so that it is easy to understand results and correlate them to your experiment setup. 

Read more

 

Advanced Guide to Working with Projects in ACAS

The ACAS database organizes experiment data (experiment parameters, raw image input, processed images, analysis data, final reports) so that it is easy to understand results and correlate them to your experiment setup. 

Read more