Posted on Jan 13, 2017

Below you will find a comparison between manual tracking and MetaVi Labs Auto-tracking. On each page, you will find an automatically generated report followed by videos of the tracked movies for each well.

The manual tracks were generated by a human using the ibidi manual track tool. These tracks were then imported into the MetaVi Labs report tool and overlaid on the movies and used to generate reports as if they were from the auto-tracker. This provides an apples-to-apples comparison.

One thing to notice is that the auto-track can follow hundreds of cells whereas the human tracker typically only follows 30 cells. So the statistical value is increased with auto-tracking.

Manual Chemotaxis Tracking Results

Metavi Labs Automated Chemotaxis Tracking Results

Posted on May 9, 2016

Q: How do I calculate pixel resolution?

A: answer here

Q: Why I am not getting any reports?

 A:  A report will not be auto-generated until all created wells have track data. In order to get track data, the input images must be first uploaded to all wells, and then the analyzer must process all wells and upload the track data. An analyzer job is created automatically when the raw data is uploaded. But it may take a few minutes to a few hours for the analyzer to get to the job depending on the work load. If you have not filled all the wells in your selected plate, you can delete empty wells. Click the report icon report icon to generate (or regenerate) a report once all the empty wells have been filled or deleted.

Q: How to I upload multiple image files to a well?

A: answer here

Q: In Wound Healing Assays, why do some (not all) of the movies uploaded not get tracked or the covered area indication is not correct?

A: The most common reason here would be a wrong Pixel per µm calculation. The Tracked Movie may find cells randomly based on cell size changes over time.  Calculating Pixel Resolution

Q: Why does my Report show 0 Value in some (not all) of the experiment wells where I would expect a lot of activity?

A-1: Please check, if your created tracked video files show a complete cell tracking (colored path). If not, please check if the µm per Pixel  settings are correct for the uploaded images or video.

A-2: Also, please check that your selected Assay type (in the experiment-set) matches your actual assay.

Q: How many different User Groups does ACAS support?

A: We allow 2 different type of Groups. “Administrators” and “Users”. Names which are participants in the “Users” group can create experiments and allow others to work on their own created projects or projects. Administrators can manage Users and other Administrators accounts. They can also manage Project owners over all “Administrators” and “Users”, no matter if they are already owner or participant of one of the projects.

Q: Why do I not receive email notifications from ACAS?

A: Your login name is an email address - please be sure to use a valid and active email address. Your local in-house ACAS contact has administrator rights which will allow the changing of email addresses. Also, check your email spam folder.

Q: How do I calculate pixel resolution?

A: answer here

Q: Why I am not getting any reports?

 A:  A report will not be auto-generated until all created wells have track data. In order to get track data, the input images must be first uploaded to all wells, and then the analyzer must process all wells and upload the track data. An analyzer job is created automatically when the raw data is uploaded. But it may take a few minutes to a few hours for the analyzer to get to the job depending on the work load. If you have not filled all the wells in your selected plate, you can delete empty wells. Click the report icon report icon to generate (or regenerate) a report once all the empty wells have been filled or deleted.

Q: How to I upload multiple image files to a well?

A: answer here

Q: In Wound Healing Assays, why do some (not all) of the movies uploaded not get tracked or the covered area indication is not correct?

A: The most common reason here would be a wrong Pixel per µm calculation. The Tracked Movie may find cells randomly based on cell size changes over time.  Calculating Pixel Resolution

Q: Why does my Report show 0 Value in some (not all) of the experiment wells where I would expect a lot of activity?

A-1: Please check, if your created tracked video files show a complete cell tracking (colored path). If not, please check if the µm per Pixel  settings are correct for the uploaded images or video.

A-2: Also, please check that your selected Assay type (in the experiment-set) matches your actual assay.

Q: How many different User Groups does ACAS support?

A: We allow 2 different type of Groups. “Administrators” and “Users”. Names which are participants in the “Users” group can create experiments and allow others to work on their own created projects or projects. Administrators can manage Users and other Administrators accounts. They can also manage Project owners over all “Administrators” and “Users”, no matter if they are already owner or participant of one of the projects.

Q: Why do I not receive email notifications from ACAS?

A: Your login name is an email address - please be sure to use a valid and active email address. Your local in-house ACAS contact has administrator rights which will allow the changing of email addresses. Also, check your email spam folder.

Posted on May 7, 2016

Web Based or In-Lab Solutions Available

Wound Healing Assay

MetaVi Labs has partnered with ibidi to automate the analysis of the ibidi Wound Healing Assays. After a few clicks of the mouse to upload your time-lapse images, the Automated Cellular Analysis System (ACAS) will provide you with a completed report.

Customers can lease access to the cloud based system, or purchase an ACAS appliance to be located in their labs.

Integrated Project Management Database

The ACAS adds the convenience of storing and managing all of your raw data, processed data, and reports in one place. Search filters make it easy to lookup your data at any time. All data is accessible via webpages. Users may upload raw data for processing from any convenient computer with a web-browser. Users are notified of completed jobs with emails which include links to the reports.

Access to project data is controlled via user accounts. Large labs can organize and control user access to critical data.

Automated Reports

View Sample Report
The ACAS will automatically processes raw image sequences from multiple wells and generates tracked movies and consolidated reports. All charts are formatted as png image files which users can then include in papers and presentations. The reports also include tab delimited raw data files.

BACKED BY 7 TERA-FLOP NUMERICAL PROCESSOR

The ACAS uses high-powered numerical processors (learn more about NVIDIA scientific computing) and innovative machine vision algorithms to provide new levels of accuracy and reliability to the read-outs.

ADVANCED IMAGE PROCESSING


MetaVi Labs designs custom image processing algorithms suitable for each specific application. In the case of wound healing, we accept a large range of images independent of cell type. The processing chain takes raw, noisy input and returns gap statistics with no intervention from the user.

The only requirement is that a gap be visible in the first frame and less than 20% of the non-gap surface area should be un-covered.

Wound Healing Automation Report - Scratch Open Area All Positions