In this sample movie, note the cell density is relatively low (preventing excessive cell-to-cell contacts).

Assay Description

Chemotaxis DF10 is optimized for Dark Field images of moving cells such as bacteria or sperm cells. Choose this version of chemotaxis assay if your image and cells have similar characteristics as the image below.

Microscopy Requirements

Microscopy Imaging Mode
Microscopy Resolution
Optimal: 2.0 pixels/µm; Minimum: 0.5 pix/µm
Field of View
Approximately 1.5 mm x 2 mm
Time Between Frames
10 seconds to 120 seconds
Image Size
Typically between 1024 and 2048 pixels wide
Image Aspect Ratio
Acceptable range is 4:3 to 16:9
Compatible Image Formats
jpg, png, bmp, tif
Compatible Video Formats
mp4, avi, mov, multi-frame-tif
Cell Density
60 / 1.0 mm2
Typical Cell Size
8.6 µm with a max extension of 20 µm

Example Image

For this example image, the typical cell is bounded by a box of 12 x 12 pixels (8 µm).

Typical Cell Size 12 x 12 pixels (8 µm)
Image Size 640 x 480 pixels (467 µm x 350 µm)
Image Resolution 1.37 pixels/µm

(This image is at the low end of the range of acceptable resolutions. The accuracy and speed of the analysis will be improved with an increased number of pixels.)

chemotaxis darkfield df10 large sample image