Dark Field microscopy is an illumination technique used to enhance the contrast in unstained samples. It works by illuminating the sample with light but only collecting the scattered light as it hits the sample, but not the light from surrounding areas. It produces dark, almost black images with brightly lit cells in high contrast.

Chemotaxis DF10

Chemotaxis DF10 is optimized for Dark Field images of moving cells such as bacteria or sperm cells. Choose this version of chemotaxis assay if your image and cells have similar characteristics as the image below.