We find that two-channel fluorescence imaging with a zoom factor of 5X to 10X and a high-resolution camera produce the best results. To achieve optimal tracking we suggest you should attempt to duplicate the sample images below in terms of contrast and resolution.
We have made excellent progress with our alpha-test partners supplying microscopy well samples. Our algorithms now track immune cells and pathogen cells when each is captured on an independent image channel (i.e. two labels).