We find that two-channel fluorescence imaging with a zoom factor of 5X to 10X and a high-resolution camera produce the best results. To achieve optimal tracking we suggest you should attempt to duplicate the sample images below in terms of contrast and resolution.

Imaging Mode fluorescence on inverted microscope
Camera Resolution 2048 pixels wide or greater
Field-of-view approximately 1 mm x 1 mm
Microscopy Resolution optimal 3.0 pix/µm, minimum: 0.5 pix/µm (calculating microscopy resolution tech note)
Glass Slide See 'Recommended Chambers' section below.
Time-lapse Recording mp4 movie file containing H.264 encoded images
Time-between-frames One frame recorded each 30 seconds

Sample Fluorescence-Based Imaging

FIBROBLASTS IN 2D COLLAGEN

Imaging Mode Dark-field, inverted microscope
Camera Resolution 1280 x 1024
Field-of-view 1,200 µm to 1,612µm (cropped from original)
Microscopy Resolution 0.623 pix/µm
Glass Slide high-quality flat bottom glass ibidi µ-Dish 35 with high resolution glass bottom

darkfield fibroblast microscopy image

BREAST CANCER CELLS IN 3D COLLAGEN

Imaging Mode Dark-field, inverted microscope
Camera Resolution 2080 x 1584
Field-of-view approximately 1 mm x 1 mm
Microscopy Resolution 2.06 pix/µm
Glass Slide standard glass slide
darkfield breastcancer microscopy cells

Sample mp4/H.264 encoded movie from the microscopy setup described above: