We find that two-channel fluorescence imaging with a zoom factor of 5X to 10X and a high-resolution camera produce the best results. To achieve optimal tracking we suggest you should attempt to duplicate the sample images below in terms of contrast and resolution.
||fluorescence on inverted microscope
||2048 pixels wide or greater
||approximately 1 mm x 1 mm
||optimal 3.0 pix/µm, minimum: 0.5 pix/µm (calculating microscopy resolution tech note)
||See 'Recommended Chambers' section below.
||mp4 movie file containing H.264 encoded images
||One frame recorded each 30 seconds
Sample Fluorescence-Based Imaging