build: commit f2381a7d Date: Wed Nov 15 05:38:36 2017 -0600

Experiment Report

Fibroblast Chemotaxis Assay Report

Information

Project
t-cell
Experiment set
t-cell
Experiment
t-cell
Experiment ID
623

Settings

Assay
Chemotaxis BF10
Plate
Custom
Cell line
un-defined
Culture medium
Substance
Evaluation interval
120 secs
Recording duration
0
Min track duration
10 secs
Movement threshold
2 µm
Time between images
9 secs
Pixel resolution
2.1807 pixel/µm
Completed on
Incubator environment:

Overview

chart num tracked cells
chart cell tracks starting
chart cell tracks ending
chart fmi
chart com displacement polar
chart com mags
chart speed excl non moving
chart speed incl non moving
chart velocity excl non moving
chart velocity incl non moving
chart directness
chart avg distance euclidian
chart accumulated distance
chart accumulated distance euclidian
chart avg cell velocity
Tabular Results, all wells:
Well Total tracks Total valid tracks Non-moving cells Moving cells Moving cells (%)
100ng-ml sdf1a003_chan00 1093 316 24 292 92.41
10ng-ml sdf1a005_chan00 1071 300 27 273 91.00
1ng-ml sdf1a006_chan00 916 206 22 184 89.32
250ng-ml sdf1a004_chan00_chan0 1463 430 56 374 86.98
500ng-ml sdf1a002_chan00 2556 424 32 392 92.45
 
Tabular Results, all wells:
Well Avg. speed, moving cells (µm/min) Avg. speed (µm/min) Avg. velocity, moving cells (µm/min) Avg. velocity (µm/min)
100ng-ml sdf1a003_chan00 3.77 3.68 3.22 3.00
10ng-ml sdf1a005_chan00 3.08 3.03 2.24 2.07
1ng-ml sdf1a006_chan00 1.96 1.95 2.06 1.89
250ng-ml sdf1a004_chan00_chan0 4.58 4.37 3.45 3.05
500ng-ml sdf1a002_chan00 4.66 4.50 4.23 3.94
 
Tabular Results, all wells:
Well Accumulated distance (µm) Mean accumulated distance (µm) Euclidean distance (µm) Mean Euclidean distance (µm)
100ng-ml sdf1a003_chan00 11667.64 38.06 6814.51 21.56
10ng-ml sdf1a005_chan00 12984.50 44.02 5748.29 19.16
1ng-ml sdf1a006_chan00 7301.40 36.28 2859.10 13.88
250ng-ml sdf1a004_chan00_chan0 16430.03 39.41 9460.29 22.00
500ng-ml sdf1a002_chan00 7450.17 19.88 6980.29 16.46
 
Tabular Results, all wells:
Well Directness FMIx FMIy CoMx (µm) CoMy (µm) Rayleigh mean
100ng-ml sdf1a003_chan00 0.64 0.02 -0.06 -0.27 -0.86 0.07
10ng-ml sdf1a005_chan00 0.54 -0.04 0.01 0.12 -1.33 0.09
1ng-ml sdf1a006_chan00 0.58 0.07 -0.17 1.85 -3.78 0.20
250ng-ml sdf1a004_chan00_chan0 0.60 0.01 -0.04 0.87 -1.85 0.06
500ng-ml sdf1a002_chan00 0.72 -0.03 0.02 0.43 0.21 0.03
 

Well Details

100ng-ml sdf1a003_chan00

Well tracking video

trajectory plot 00
velocity histogram plot 00
velocity histogram percent plot 00
track length histogram plot 00
chart endpoint polar 00
Tracking data:
Total cells
316
FMIx
0.02
FMIy
-0.06
CoMx
-0.27 µm
CoMy
-0.86 µm
Avg. speed, moving cells
3.77 µm/min
Avg. speed
3.68 µm/min
Avg. velocity, moving cells
3.22 µm/min
Avg. velocity
3.00 µm/min
Directness
0.64
Accumulated distance
11667.64 µm
Euclidean distance
6814.51 µm
Rayleigh mean
0.07
 

10ng-ml sdf1a005_chan00

Well tracking video

trajectory plot 01
velocity histogram plot 01
velocity histogram percent plot 01
track length histogram plot 01
chart endpoint polar 01
Tracking data:
Total cells
300
FMIx
-0.04
FMIy
0.01
CoMx
0.12 µm
CoMy
-1.33 µm
Avg. speed, moving cells
3.08 µm/min
Avg. speed
3.03 µm/min
Avg. velocity, moving cells
2.24 µm/min
Avg. velocity
2.07 µm/min
Directness
0.54
Accumulated distance
12984.50 µm
Euclidean distance
5748.29 µm
Rayleigh mean
0.09
 

1ng-ml sdf1a006_chan00

Well tracking video

trajectory plot 02
velocity histogram plot 02
velocity histogram percent plot 02
track length histogram plot 02
chart endpoint polar 02
Tracking data:
Total cells
206
FMIx
0.07
FMIy
-0.17
CoMx
1.85 µm
CoMy
-3.78 µm
Avg. speed, moving cells
1.96 µm/min
Avg. speed
1.95 µm/min
Avg. velocity, moving cells
2.06 µm/min
Avg. velocity
1.89 µm/min
Directness
0.58
Accumulated distance
7301.40 µm
Euclidean distance
2859.10 µm
Rayleigh mean
0.20
 

250ng-ml sdf1a004_chan00_chan0

Well tracking video

trajectory plot 03
velocity histogram plot 03
velocity histogram percent plot 03
track length histogram plot 03
chart endpoint polar 03
Tracking data:
Total cells
430
FMIx
0.01
FMIy
-0.04
CoMx
0.87 µm
CoMy
-1.85 µm
Avg. speed, moving cells
4.58 µm/min
Avg. speed
4.37 µm/min
Avg. velocity, moving cells
3.45 µm/min
Avg. velocity
3.05 µm/min
Directness
0.60
Accumulated distance
16430.03 µm
Euclidean distance
9460.29 µm
Rayleigh mean
0.06
 

500ng-ml sdf1a002_chan00

Well tracking video

trajectory plot 04
velocity histogram plot 04
velocity histogram percent plot 04
track length histogram plot 04
chart endpoint polar 04
Tracking data:
Total cells
424
FMIx
-0.03
FMIy
0.02
CoMx
0.43 µm
CoMy
0.21 µm
Avg. speed, moving cells
4.66 µm/min
Avg. speed
4.50 µm/min
Avg. velocity, moving cells
4.23 µm/min
Avg. velocity
3.94 µm/min
Directness
0.72
Accumulated distance
7450.17 µm
Euclidean distance
6980.29 µm
Rayleigh mean
0.03